Rabbit Monoclonal Antibody Production

An advanced single cell screening and cloning technology is used to enhance yield and specificity of rabbit mAbs.

ABclonal provides a customized rabbit recombinant monoclonal antibody service using proprietary B cell cloning technology. This unique technology employs innovative cell sorting, enriching, bioinformatics and advanced cloning. It does not take the traditional approach of using the hybridoma method, hence avoiding the stability issue associated with hybridomas.

The rabbit monoclonal technology has a high success rate towards difficult targets such as post-translational modification site, small molecule, and proteins with point mutation.

Our rabbit monoclonal service provides

  • One-stop service – from consultation, antigen design and synthesis to antibody purification and validation.
  • Flexible optimization at each stage.
  • Both monoclonal antibodies and polyclonal antibodies if requested.
  • Claims no Intellectual Property for the resulting antibody product.

Highlights

  • High affinity
  • Superior specificity
  • Broad epitope recognition
  • Good recognition of small molecules and post translational modification sites
  • Multiple applications, including IF, IHC, ChIP, ELISA, Flow, IP and WB
  • Perfect choice for testing rodent proteins and tissues
  • One major IgG isotype which makes it easier to clone

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.

Rabbit Monoclonal Antibody Production Process

Overview of standard animal immunization schedules

Deliverables:

100 μg of up to five different recombinant rabbit monoclonal antibodies (ELISA positive)

Full-length antibody genes of selected clone

Clones in plasmid format

Be the next success story.

Case Study 1: First Rabbit mAbs Against CCL18

Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.

  • 1200 single B cell clones
  • 119 positive clones (10%)
  • 26 selected clones
  • 24 pairs of mAb genes
  • 22 functional mAbs
  • 17 specific anti-CCL18 mAbs
  • 6 multi-assay mAbs
Case Study 2: Efficient Screening Of Anti-Hapten Rabbit mAbs

Rabbits are better than mice in presenting nonpeptic haptens because of their expression of proteins in the CD1 family. For this project, results achieved include:

  • 1334 single B cell clones
  • 100 positive clones (7.4%)
  • 18 selected clones
  • 17 pairs of mAb genes
  • 16 functional mAbs
Case Study 3: Quick Discovery Of PD-L1-Blocking Antibodies

PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include:

  • 2304 single B cell clones
  • 386 positive clones (16.8%)
  • 19 strong PDL1-blocking clones (4.9%)
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